DNase I Reaction Buffer

产品编号：B0303S 品牌：New England Biolabs 原厂货号：B0303S
产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
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包装：

运保温度:

-20°C

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描述：

Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.

Reagents Supplied:
10X DNase I Reaction Buffer

1X DNase I Reaction Buffer:
10 mM Tris-HCl
2.5 mM MgCl₂
0.5 mM CaCl₂
pH 7.6 @ 25°C

RNase Activity:
Incubation of DNase I at 1X concentration with 100 units of DNase I and 10 μg of double-stranded RNA Ladder for 2 hours at 37°C resulted in the same electrophoretic profile as untreated RNA.

Incubation of DNase I at 1X concentration with 2 units of DNase I and 10 μg of single-stranded RNA Ladder for 1 hour at 37°C resulted in the same electrophoretic profile as untreated RNA.