ChromaLink? Digoxigenin One-Shot Antibody Labeling Kit

产品编号：B-9014-009K 品牌：solulink 原厂货号：B-9014-009K
产品分类：生化和细胞分析 > 标签、标记试剂盒 > 抗体、蛋白标记试剂盒
应用分类：

包装：

Labels 100 μg of Ab

运保温度:

room temperature

到货周期：

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其它组分：

Sulfo ChromaLink TM Digoxigenin【包装：11 µg /1，，运保温度：room temp】

1x Modification Buffer (pH 8.0)【包装：1.5 ml /1，，运保温度：room temp】

1x PBS (pH 7.2)【包装：1.5 ml /1，，运保温度：room temp】

DMF (anhydrous)【包装：500 µl/1，，运保温度：room temp】

Collection tubes【包装：1.5 ml /4，，运保温度：room temp】

Zeba TM Spin Columns【包装：0.5 ml /2，，运保温度：room temp】

Digoxigenin-IgG Control【包装：100 µg /1，，运保温度：room temp】

1M Tris.HCL (pH 8.9)【包装：500 µl /1，，运保温度：room temp】

描述：

Single-reaction labeling kit with measurable chromophore
The ChromaLink™ Digoxigenin One-Shot Antibody Labeling Kit provides convenient, measurable digoxigenin labeling of 100 µg of antibody. The kit contains Sulfo ChromaLink™ Digoxigenin, which incorporates a UV-traceable chromophore, based on Solulink’s proprietary linking technology, to enable reproducibility in your labeling process. With a simple and direct UV scan (A₂₈₀ and A₃₅₄), you can quantify digoxigenin incorporation and ensure reproducible production of consistent batches. A large scale kit is also available (labels 2 mgs of antibody).

Workflow Diagram

The ChromaLink™ Digoxigenin One-Shot Antibody Labeling Kit includes buffers, spin columns, and even a calculator to determine MSR. With the ChromaLink™ Digoxigenin One-Shot Antibody Labeling Kit, you can label and purify 100 µg of antibody or proteins ranging in molecular weight from 20–200 kDa in about 2 hours.

The first measurable digoxigenin reagent
Digoxigenin has long been used as an alternative hapten reporter system to the streptavidin/biotin system. In recent times, this reporter system has also been used side-by-side with the biotin-streptavidin system for the detection of multiplexed antigens. Unlike the biotin reporter system, digoxigenin incorporation has never had any method of quantifying incorporated label like the biotin HABA assay, until now. ChromaLink™ Digoxigenin Reagent features high antibody recoveries (50–80%) with consistent digoxigenin incorporation. The ideal degree of incorporation, or molar substitution ratio (MSR), ranges from 2–8 digoxigenin molecules per antibody.

ChromaLink™ Digoxigenin One-Shot Antibody Labeling Kit Advantages

Reproducible Production – UV-traceable chromophore permits nondestructive, rapid A₂₈₀/A₃₅₄ quantification of incorporated digoxigenin, enabling consistency and reproducibility
No toxic DMF or DMSO – Amine-reactive sulfo-succinimidyl ester is safe and water-soluble, avoiding the use of organic solvents
Extended PEG3 Spacer – Helps reduce aggregation, minimizes steric hindrance, and enhances solubility
One-shot – All components sized to quickly label 100 µg of antibody
Convenient – Kit contains buffers, spin columns, and calculator to determine MSR

Product Formats

ChromaLink™ Digoxigenin One-Shot Antibody Labeling Kit provides quantitative digoxigenin labeling of 100 µg of antibody (Cat. No. B-9014-009K)
ChromaLink™ Digoxigenin Reagent is available in 5 × 0.5 mg and 0.5 mg sizes (Cat. No. B-1014-505 or B-1014-005)