Overview
• No monitoring cell density
• Automatic induction of protein expression
• High protein yield
Detailed Description
AMRESCO's ZYP-5052, Autoinduction Media is formulated with quality biochemicals to ensure consistent growth and protein expression using methodology developed by Dr. Studier at Brookhaven National Laboratory. ZYP-5052, Autoinduction Media activates recombinant protein expression in E. coli through diauxic growth, with the first growth phase producing a high density bacterial culture. During the second phase, lac promoters are activated and result in induction of prolific transcription and translation of the cloned DNA. A significant percentage of the total cell protein becomes comprised of recombinant DNA, which can then be isolated and purified for downstream applications.
A protein expression protocol using ZYP-5052, Autoinduction Media is more effiecient than conventional methods for both high throughput routine protein expression, because it eliminates the need for cell density and the manual addition of IPTG for induction. An additional benefit of using ZYP-5052, Autoinduction Media is improved protein yield compared to the yield achieved by a conventional growth and induction method.
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