AHP873 is specific for human Aurora A Kinase, also known as Serine/Threonine Kinase 6 (STK6).
Aurora A Kinase is a cell-cycle regulated kinase that has an important role in centrosome maturation and chromosome segregation. This protein is found at the centrosome in interphase cells and at the spindle poles in mitosis.
Recent studies have revealed that Aurora A Kinase is frequently overexpressed in various human carcinomas, which would suggest that this protein may be involved in tumorigenesis.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/250 - 1/1000
Immunohistology - Paraffin
Immunoprecipitation
2ug/mg protein - 10ug/mg protein
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human Aurora A Kinase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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