This product, a Goat anti Guinea Pig IgG (H/L): HRP antibody, recognizes guinea pig IgG (heavy and light chains). This antibody has been shown to react specifically with guinea pig IgG and with light chains common to other guinea pig immunoglobulins, by immunoelectrophoresis and ELISA.
This antibody may cross react with IgG from other species.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/1000 - 1/10,000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to Guinea pig IgG (H & L) were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using antigen coupled to agarose beads.