Goat anti Human BD-2 antibody recognizes human defensin beta-2, a 4.3kDa cationic antimicrobial peptide expressed primarily by epithelial cells of the respiratory tract and skin, and an important component of the innate immune response against microbial infections.
Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-terminal active portion of the protein.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0 ug/ml
Flow Cytometry
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2 ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
This biotinylated human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions withrecombinant human BD-2(PHP161) as the positive control.
Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This biotinylated human DB-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with apurified human BD-2 antibody(AHP849) as the capture reagent andrecombinant human BD-2(PHP161) as the standard.
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