AHP804 is specific for human calpain-9. Calpain-9 is a calcium activated protease, which is expressed in the stomach and digestive tract cells. Calpain-9 large subunit is made up of four domains. AHP804 recognises an EF hand (calcium binding) region within domain IV. The antibody can be used to detect both latent and active forms of calpain-9.
Calpain-9 is thought to act as a tumour suppressor for gastric cancer. For some gastric cancers a down regulation of calpain-9 has been reported, in other gastric cancers shorter forms of calpain-9 with deletions in domain II have been reported.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own using appropriate negative/positive controls.
Western Blotting
Under reducing conditions AHP804 detects major bands of 79kD and 58kD.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human calpain-9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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