AHP795B is specific for human IL-10 (Interleukin-10), an 18.5kDa immunosuppressive cytokine originally known as cytokine synthesis inhibiting factor (CSIF), which specifically regulates immune-mediated inflammation and shares high sequence homology with murine IL-10 and the viral protein BCRF1.
IL-10, produced by macrophages, mast cells, T cells, activated B cells and keratinocytes, inhibits the secretion of a variety of pro-inflammatory cytokines including TNFalpha, IL-1, IL-2, IL-3 and IL-8 and is chemotactic for CD8+ T cells. The suppressive ability of IL-10 has become a focus area for the treatment of Crohn’s disease, but with conflicting results.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
