AHP767B is specific for mouse EGF (Epidermal Growth Factor), a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, which is expressed by a variety of cells.
The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a, VGF (vaccinia virus growth factor) and Betacellulin, produced by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.15 - 0.30ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
AHP767B may be used in an direct ELISA system or as a detection antibody in a sandwich ELISA for human EGF in combination withAHP767as a capture reagent.
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