AHP766B is specific for human BCA-1 (B cell attractant chemokine), a 10.1kDa CXC cytokine designated CXCL13, which acts as a potent chemoattractant for B lymphocytes, and is expressed at high levels in the liver and to a lesser extent by lymph nodes, spleen, stomach, appendix and salivary glands.
Studies have revealed that BCA-1 functions as a ligand for Burkitt’s lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human CXCL13 were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG was prepared from whole serum by affinity chromatography.
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