Goat anti Human SMAD2 antibodyrecognizes human SMAD2, also known as MADH2 or Mothers against decapentaplegic homolog 2. SMAD2 is a 467 amino acid ~50kDa intracellular signaling protein responsible for mediating the signal from TGFβ. SMAD2 associates woth the TGFβ receptor via the SMAD anchor for receptor activation (SARA) and on binding of TGFβ to the receptor SMAD2 is phosphorylated and dissociates from SARA. SMAD2 then forms an association with another SMAD family member SMAD4, important for translocation to the nucleus where it binds to its target promoters and activates TGFβ induced pathways.
There are two identified isoforms of SMAD2 with the short isoform lacking an internal sequence from amino acid 79 to amino acid 108. The immunogen sequence for Goat anti Human SMAD2 antibody is contained within this region and hence this antibody is only expected to recognize the long, canonical isoform of SMAD2..
SMAD2 is expressed at high levels in placenta skeletal muscle and heart (Xuanet al.2007)
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/200 - 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP694 detects bands of approximately 45kDa and 50kDa in HeLa cell lysates. a minimum incubation time of 1 hour is recommended with this antibody
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to MADH-2 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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