AHP669 recognises the protein designated GAP43, a neuronal protein that is expressed at elevated levels during development and also during regenerative axon growth.
GAP43 (also known as neuromodulin, pp46, B-50, Protein F1 and calmodulin-binding protein P-57) is a protein of approximately 25kD that migrates on SDS-PAGE gels with an apparent size of 40-50kD. The aberrant migration is due to the unusually low abundance of hydrophobic amino acid residues. GAP43 is found tightly associated with neuronal membranes due to palmitoylation. The solubilised protein binds calmodulin, although this is reduced following protein kinase C phosphorylation of GAP43.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Yes
Monkey
Yes
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/500 - 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to GAP43 were raised by repeated immunisations of rabbits with highly purified antigen.
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