AHP535 recognizes both the wild type and mutant forms of p53. The oncoprotein p53 is an intensively studied tumour suppressor protein, which has a vital role in the control of cell growth.
In normal circumstances cells arrest during the G1/G2 phase of the cell cycle, and undertake any DNA repair processes that are necessary. This arrest is mediated by p53, and if p53 is absent or damaged cells with cancerous potential may be able to continue through the cell cycle.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
1/10 - 1/40
Immunohistology - Paraffin(1)
1/10 - 1/40
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human p53 were raised by repeated immunisation of rabbits with highly purified antigen.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
