AHP457 recognises human alpha fetoprotein, and shows positive staining of parenchymal cells of fetal liver and neoplastic liver cells showing yolk sac differentiation in germ cell tumours.
Species Cross-Reactivity
Target Species
Cross Reactivity
Pig
Yes
Sheep
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
1/10 - 1/40
Immunohistology - Paraffin
1/10 - 1/40
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Storage
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human AFP were raised by repeated immunisation of rabbits with highly purified antigen
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