AHP431 recognises the 59kD apoptosis associated protein designated Caspase-10beta.
Caspase-10 is a novel ICE/CED-3 protease, and cleaves and activates Caspase -3, -4, -6, -7, -8 and -9 leading to the cleavage of many key proteins such as PARP.
Caspase-10 exists in two forms that differ in sequence at the C-terminus. Caspase-10 is also known as Mch4, whilst Caspase-10beta is also known as FLICE2.
This antibody only recognises the FLICE 2 form of Caspase-10.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/500 - 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Caspase-10 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
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