Sheep anti Human vitronectin recognises a plasma protein of 75kDa, vitronectin also known as complement S-protein, V75 or serum spreading factor, which may exist in either the V75 form or the clipped V65 (65kDa) +V10 (10kDa) form comprising 2 chains linked by a disulphide bond.
Vitronectin is a multifunctional protein, being a complement lysis inhibitor and also a scavenger protein for macromolecular products of the complement and homeostasis cascades.
Vitronectin promotes attachment and spreading of various animal cell typesin vitro.Kubotaet al.classified human blood samples into 3 distinct vitronectin profile types, type 1 rich in intact V75 (75kDa) polypeptide and poor in its nicked product V65 (65kDa), type 2, containing roughly equal amounts of V75 and V65 and type 3, poor in V75 and rich in V65. These types comprised approimately 58, 35 and 5% of the studied population respectively. The vitronectin blood type showed no correlation with age, sex or ABO blood group.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunodiffusion
Neat
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human vitronectin were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
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