AHP392 recognises the human ATM protein, a 350kD polypeptide that is the product of the ATM gene. The ATM gene is defective in Ataxia telangiectasia.
ATM protein is expressed within the nucleus of all normal cells and is thought to be important in detection of DNA damage.
Species Cross-Reactivity
Target Species
Cross Reactivity
Hamster
Yes
Monkey
Yes
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
1/50
Immunoprecipitation
Western Blotting
1/500 - 1/1000
(1)
This product requires heat mediated treatment of paraffin sections. Serotec recommend the use of antigen unmasking fluid for this purpose. A two hour incubation with AHP392 is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to ATM were raised by repeated immunisation of rabbits with highly purified antigen.
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