AHP288 is reactive with human NFkB p65 subunit (Rel A) .
In a gel supershift assay this antibody was found to be active against all p65 containing human, mouse or rat NFkB complexes using 0.5ul to 1.0ul per assay.
The specific binding of this antibody to the NFkB p65 subunit can be blocked using the NFkB p65 control peptide(PHP073).
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/5000 - 1/25000
Flow Cytometry
Gel Super Shift Assays
0.5 - 1.0 ul per test
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/2000 - 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to NFkB p65 were raised by repeated immunisation of rabbits with highly purified antigen.
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