AHP2253 specifically recognises an epitope within the N-Terminal (NT) region of human MAML1 (Mastermind-like Protein 1), a widely expressed nuclear protein and member of the mastermind family, which acts as a crucial coactivator of Notch-mediated transcriptional activation, and plays a role in hematopoietic development, by acting as a regulator of Notch-mediated lymphoid cell fate decisions.
MAML1 interacts with the Mediator complex component CDK8 (Cyclin-dependent kinase 8), enhancing the phosphorylation and proteolytic turnover of the Notch intracellular domain (NICD) within the nucleus, and forms a DNA-binding complex with RBPSUH/RBP-J kappa/CBF1, playing a vital role in the transcriptional activation of Notch targets. Studies have shown that the NT region of MAML1 interacts with the DNA-binding domain of the tumor suppressor protein p53, and that over-expression of MAML1, greatly increases p53-mediated gene transcription and biological function.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/128,000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2253 detects a band of approximately 110kDa in human skeletal musde cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human MAML1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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