AHP2216 recognises Alpha-adaptin (AP2A2), a component of the adaptor protein complex 2, which plays a role in protein transport in various membrane traffic pathways.
AP2A2, together with NUMB/NUMBL, plays a role in the endosomal degradation and recycling of Notch receptors, during the Notch signaling pathway.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/1000
Flow Cytometry
1/10 - 1/50
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
1/50 - 1/100
Immunoprecipitation
Western Blotting
1/100 - 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to human Alpha-adaptin were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by protein A chromatography, followed by affinity purification.
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