RABBIT ANTI HUMAN GLUT-1 (C-TERMINAL)

产品编号：AHP2173 品牌：AbD Serotec 原厂货号：AHP2173
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

50 μg

运保温度:

+4oC or -20oC

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描述：

Specificity

AHP2173 specifically recognises an epitope within the C-Terminal (CT) region of GLUT-1 (Glucose transporter 1), a ubiquitously expressed integral membrane glycoprotein, and member of the major facilitator superfamily, which plays a major role in the transport of glucose across the cell membrane, and the blood-brain barrier.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Yes
Rat	Yes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin⁽¹⁾				1;100 -
Western Blotting				1:500 - 1:2000

(1)

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting

AHP2173 detects a band of approximately 54kDa in human Jurkat cell lysates.

Storage

Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Antiserum Preparation

Antiserum to human GLUT-1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.