AHP2157 recognises Human dioxin inducible Cytochrome P450 1B1 (CYP1B1), a catalytic protein belonging to the Cytochrome P450 family, a group of heme-thiolate mono-oxygenases.
CYP1B1 is involved in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development, defects in CYP1B1 are a cause of various eye defects including Peters anomaly, POAG and GLC3A.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Chimpanzee
Expected from Sequence
Dog
Expected from Sequence
Horse
Expected from Sequence
Monkey
Expected from Sequence
Mouse
Yes
Rat
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
10ug/ml -
Immunoprecipitation
Western Blotting
0.5 - 1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to CYP1B1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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