AHP2140 specifically recognises an epitope within the C-Terminal (CT) region of human Exosome component 9 (EXOSC9), otherwise known as PMSCL1.
EXOSC9 is a member of the RNase PH family, and component of the exosome 3'->5' exoribonuclease (PM/Scl) complex, which exhibits exonuclease activity, and is involved in the degradation of unstable RNA, and the 3' processing of the 7S pre-RNA to the mature 5.8S rRNA . EXOSC9 has been identified as an autoantigen in patients with the complex auto immune disease scleromyositis.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/64000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.3 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2140 detects a band of approximately 75kDa in Jurkat cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human EXOSC9 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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