AHP2045 recognises porcine Glutamate oxaloacetate transaminase 2 (GOT2), also widely known as Aspartarte transaminase, a 47.7kDa protein belonging to the class-I pyridoxal-phosphate-dependent aminotransferase family.
GOT2 is involved in the transport of lipids across cell membranes, three isoenzymes are known, cytoplasmic, mitochondrial and chloroplastic, AHP2055 recognises the mitochondrial form.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
20ug/ml -
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to porcine GOT2 was raised by repeated immunisation of sheep with highly purified antigen. Ig fraction was prepared from whole serum by ammonium sulphate precipitation.
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