AHP1869 recognises N-ethylmaleimide sensitive fusion protein (NSF), a trimeric ATPase component of the SNARE protein complex. It catalyses the fusion of transport vesicles within the Golgi cisternae and is also involved in transport from the endoplasmic reticulum to the Golgi stack. NSF seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack regardless of vesicle origin.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Expected from Sequence
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1869 detects a band of approximately 75kDa in rat caudate lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to NSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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