AHP1818 specifically ecognises an epitope within the N-terminal (NT) region of human MEK7, otherwise known as MAPKK7 (mitogen activated protein kinase kinase 7), a ubiquitously expressed dual specificity protein kinase, and member of the tyrosine/threonine protein kinase family, which acts as a signal transducer in response to environmental stresses/inflammation.
MEK7 is activated by phosphorylation by specific kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK, and is an activator of the Jun kinases JNK1/MAPK8, JNK2/MAPK9 and JNK3/MAPK10 signalling pathways.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/100000
Flow Cytometry
Immunofluorescence
1/300
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human MEK7 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
ELISA
This product is suitable for use in indirect ELISA applications.
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