RABBIT ANTI RIG-I

产品编号：AHP1776T 品牌：AbD Serotec 原厂货号：AHP1776T
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

50 μg

运保温度:

+4oC or -20oC

到货周期：

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描述：

Specificity

AHP1776T recognises human Retinoic acid-inducible gene 1 protein (RIG-I), also known as RIG-1 or DEAD-box protein 58. RIG-I is a receptor found in the cytoplasm that recognises viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Yes
Rat	Yes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
Immunohistology - Paraffin⁽¹⁾				5.0ug/ml
Western Blotting				0.5 - 1.0ug/ml

(1)

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting

AHP1776T detects a band of approximately 84 kDa in C2C12cell lysate.

Storage

Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Antiserum Preparation

Antisera to human RIG-I were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.