AHP1722 recognises the long isoform of human Bcl-2-like protein 1, otherwise known as Bcl-xL, located on the mitochondrial membrane. The Bcl-xL isoform (the longer isoform) is a potent inhibitor of apoptosis. It appears to regulate cell death by binding to and blocking the voltage-dependent anion channel, thereby preventing the release of the caspase activator cytochrome c. High expression of Bcl-xL in tumours correlates to advanced disease and a poor prognosis.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
1 - 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1722 detects a band of approximately 27kDa in A549 cell lysate
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to Bcl-xL was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
