AHP1720 recognises the C-terminal of human High temperature requirement protein A2 (HtrA2), isoform 1. It is thought to play a role in cellular stress response as it is up-regulated following heat shock and endoplasmic reticulum stress. It promotes cell death by binding and inactivating inhibitor of apoptosis proteins (IAPs), which contain baculoviral IAP repeat (BIR) binding domains. This causes an increase in caspase activity. Isoform 1 (45kDa), but not isoform 2 (35kDa) can also proteolytically cleave IAPs. Additionally HtrA2 can induce cell death via a mechanism dependent on its serine protease activity that is independent of IAP and caspases. Defects in HtrA2 are linked to Parkinson disease 13. HtrA2 is localised to the intermembrane space of the mitochondria to be released into the cytoplasm in response to apoptotic stimuli.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Paraffin(1)
10ug/ml
Western Blotting
0.5 - 1.0ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1720 detects a band of approximately 45kDa in human colon tissue lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to HtrA2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
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