AHP1712T recognises human BR serine-threonine kinase 1 (BRSK1), a cytoplasmic and nuclear protein belonging to the protein kinase super family.
BRSK1 is a widely expressed protein, with highest levels being detected in the brain and testis. It is essential for the polarization forebrain neurones which results in the distinct properties of axons and dendrites.
Research suggests that BRSK1 is involved in the regulation of G2/M arrest and functions as a checkpoint kinase following UV- or methyl methane sulfonate (but not IR-) induced DNA damage. BRSK1 phosphorylates WEE1 and CDC25Bin vitroand CDC25Cin vitroandin vivo.
Multiple isoforms of BRSK1 have been identified.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Paraffin(1)
2.5ug/ml
Western Blotting
0.5 - 1.0ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1712T detects a bands of approximately 87kDa in human brain tissue lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Human BRSK1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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