AHP1658T recognises human ARTS (apoptosis related protein in TGF-beta signalling pathway), also known as septin-4. It is a member of the septin family, evolutionary conserved proteins involved in cytokinesis. ARTS is expressed ubiquitously in adult and foetal tissues, in particular in the heart, liver and adrenal gland. It is also expressed in colorectal cancer and malignant melanoma. ARTS enhances cell death induced by TGF-beta and is found in the mitochondria until apoptosis occurs, when it moves to the nucleus.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Paraffin(1)
2ug/ml
Western Blotting
0.5 - 2.0ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1658T detects a band of approximately 32kDa in lung lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to ARTS was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
