AHP1621 recognises human interleukin-22 receptor (IL-22R), also known as Interleukin-22 receptor alpha, a member of the class II cytokine receptor family. It associates with the Beta chain of the Interleukin-10 receptor to form the functional IL-22 receptor that enables IL-22 signalling via the JAK-STAT signalling pathway. It also forms a part of the receptors for IL-20 and IL-24.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1621 detects a band of approximately 62kDa in HepG2 cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human IL-22R were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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