AHP1549 recognises rat metabotropic glutamate receptor 2 and 3 (mGluR2 and mGluR3). The metabotropic glutamate receptors have been organised into three groups, mGluR2 and mGluR3 make up group II, and are negatively coupled to adenylate cyclase. Group II mGluRs act to inhibit neurotransmitter release as autoreceptors on glutamatergic terminals and as presynaptic heteroreceptors, performing a variety of functions in the central and peripheral nervous system. Studies have shown that mGluRs play an important role in neuroplasticity and group II mGluRs are targeted by various drugs used for treating anxiety, Parkinson’s disease, schizophrenia and drug addiction.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Human
Expected from Sequence
Mouse
Expected from Sequence
Primate
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/500
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1549 detects bands of approximately 110kDa (monomer) and 220kDa (dimer) in rat hippocampal lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to metabotropic glutamate receptor 2/3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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