AHP1360 recognizes an epitope within the common antigen (VP6) of both human and animal group A rotavirus (rotavirus A), the most common causative viral agent of severe gastroenteritis among the young worldwide, resulting from the production of a powerful diarrhoea-inducing enterotoxin, within the small intestine.
The serotypes of rotavirus A are determined by two gene products: protease-sensitive protein VP4 and glycoprotein VP7, which act as antigens on the surface of the virus, and are used to classify group A rotavirus as either P type or G type, respectively.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to human group A rotaviruses were raised by repeated immunisations of rabbits with highly purified antigen.
ELISA
AHP1360 can be used as both a capture and conjugated detection antibody in ELISA.
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