AHP1351 detects estrogen-related receptor gamma (ERR gamma), a member of the nuclear hormone receptor superfamily. The fasting-induced coactivator protein PGC-1a has been shown to activate the ERR gamma, and together these proteins have a major role in the regulation of ERR alpha gene expression and energy homeostasis.
ERR gamma and alpha are co-expressed in high energy demanding tissues such as skeletal muscle heart and kidney, and have been suggested to function in their differentiation.
ERR gamma has also been shown to be over-expressed in breast tumours, and has been suggested to be a potential biomarker for favourable clinical course and hormonal sensitivity.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human ERR gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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