AHP1388 detects human inhibitor of apoptosis stimulating protein of p53 (iASPP), which plays a central role in regulation of apoptosis and transcription via its interaction with NFkB and p53 proteins. At present, the iASPP gene is thought to encode three isoforms; isoform 1 is predominantly located in the cytoplasm, whilst isoforms 2 and 3 are nuclear proteins. iASPP is highly expressed in the heart, placenta and prostate.
iASPP has been shown to have oncogenic properties in human breast carcinomas.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/40000 - 1/160000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000 - 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1338 detects a bands of approximately 100 and 50kDa corresponding to iASPP isoforms 1 and 2 respectively.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human iASPP were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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