AHP1275 is specific for human Artemin, a disulfide-linked homodimer and member of the GDNF (glial cell line-derived neurotrophic factor) family of ligands, and cysteine-knot superfamily of growth factors, which shares structural homology with GDNF, Persephin and Neurturin.
Artemin acts as a neurotrophic survival and differentiation factor for all peripheral ganglia, and mainly signals through binding to the GDNF-family receptor known as GFR-alpha-3, which then forms a complex with the tyrosine kinase transmembrane signalling receptor RET.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.5 - 2.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human Artemin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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