AHP1248 detects an epitope within the C-terminal region of human TAP1, also known as transporter associated with antigen processing 1. TAP1 is an 81kDa membrane-associated protein which is a member of the ATP-binding cassette (ABC) transporter superfamily and together with the homologous subunit TAP2 forms the functional TAP complex.
TAP1 is involved in the transport of antigenic peptides from the cytosol into the endoplasmic reticulum, where they associate with major histocompatibility complex (MHC) class I molecules. These complexes are presented at the cell surface to T-lymphocytes, which identify and remove infected or malignantly transformed cells.
AHP1248 does not cross react with TAP2.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/10000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1248 detects a band of approximately 72kDa
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to TAP1 (C-terminal) were raised by repeated immunisations of rabbits with highly purified antigen.
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