AHP1227 detects an epitope within the N-terminal region of human CD61, also known as integrin beta 3 (ITGB3). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. CD61 is a 90-110 kDa glycoprotein which binds to CD41 (ITGA2B) to form the GPIIb/IIIa complex, and CD51 (ITGAV) to form the vitronectin receptor.
Binding of fibrinogen to the GPIIb/IIIa complex brings about platelet/platelet interaction, leading to rapid platelet aggregation and clotting. The vitronectin receptor is essential for endothelial cell migration, proliferation, and prevention of apoptosis.
Elevated expression of the vitronectin receptor is associated with tumour progression and metastases formation on melanoma cells, and is used by human cytomegalovirus (HCMV) as a co-receptor to initiate viral entry and signalling.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
10 - 20ug/ml
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human CD61 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
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