AHP1223 detects an epitope within the extracellular domain of human CD49b, also known as integrin alpha-2 (ITGA2). CD49b is a 160kD glycoprotein that non-covalently associates with integrin beta 1 (CD29) to form the VLA-2 complex. This complex is involved in cell adhesion and cell-surface mediated signalling, and functions as a cell surface receptor for many types of collagen. Originally identified on activated human T lymphocytes, this complex was subsequently shown to be present on a wide variety of cell types including fibroblasts and platelets.
VLA-2 has been linked to cardiovascular disease, cancer development and metastasis. CD49b has been identified on a subset of regulatory T cells which produce interleukin 10 (IL-10).
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
5 - 15 ug/ml
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human CD49b were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
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