AHP1190 specifically recognises an epitope within the C-Terminal (CT) region of nicastrin, a widely expressed type I transmembrane glycoprotein and member of the nicastrin family, which is constitutively expressed by neuronal cells, and is an essential component of the endoprotease gamma-secretase complex.
The gamma-secretase complex, consisting of APH-1 (APH-1A or APH-1B), presenilin-1 or -2 (PSEN1 or PSEN2), nicastrin (NCSTN), and Pen-2 (PEN2), plays a vital role in the evolutionarily conserved Notch signaling pathway, by catalyzing the intramembrane S3 cleavage of ligand-activated Notch receptor proteins (Notch1-4), resulting in the generation and release of an activated NICD fragment. Nicastrin is also involved in the cleavage of the beta-amyloid precursor protein, to form the amyloid beta peptide, a major component of the neuritic plaques found in Alzheimer’s disease.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
5.0ug/ml
Immunoprecipitation
Western Blotting
0.5 - 2.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1190 detects a band of approximately 78kDa in mouse brain tissue lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human nicastrin (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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