AHP1177 detects an epitope within the C-terminal (CT) region of human C1q and TNF related protein 6 (CTRP6), also known as C1QTNF6. CTRP6 is an adipokine; proteins which are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism.
CTRP6 is highly post-translationally modified, containing at least 4 glycosylation motifs. It has a predicted molecular weight of 29kD, and an observed molecular weight of 42kD.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
10ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1 - 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1177 detects a band of approximately 42kDa in heLa cell lysates. These proteins often migrate in SDS-PAGE at positions other that their predicted size.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human CTRP6 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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