AHP1147 detects an epitope within the N-terminal (NT) region of phosphodiesterase 2A (PDE2A), a 100kD cyclic nucleotide phosphodiesterase (PDE). Three isoforms of PDE2A have been reported (PDE2A1, PDE2A2, and PDE2A3) which arise from a single gene by alternative splicing events. PDE2A can degrade both cAMP and cGMP, but degrades cGMP with greater efficacy.
PDE2A is expressed in a variety of tissue types, with greatest expression in the brain, where it is localised in the cerebellum and neocortex. PDE2A has been implicated in penile erectile dysfunction and in the regulation of fluid and inflammatory cell transport across the endothelial cell barrier.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
No
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
15 - 25ug/ml
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to phosphodiesterase 2A (NT) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
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