AHP1096 detects an epitope corresponding to 429-442aa of human LXR (liver X receptor) alpha and to 443-456aa of human LXR beta. LXR alpha and beta are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors, and each have a molecular weight of 55kD.
LXR alpha and beta are sterol-responsive with the ability to directly bind several oxysterol metabolites. Many LXR target genes have been identified that implicate these receptors in a variety of physiological processes including cholesterol transport and metabolism, glucose metabolism, and inflammation.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1 - 3ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1096 detects a band of approximately 55kDa in human spleen lysates. A minimum incubation time of 1 hour is recommended with this antibody.
* Additional minor bands of 38 and 75kD may also be present, these are blocked by pre-incubation of the antibody with the immunising peptide.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human LXR Alpha/Beta were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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