AHP1062 detects an epitope within the N-terminal (NT) of extracellular signal-regulated kinase 1 (ERK1). ERK1 is a 45kD protein involved in the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. ERK1 is a member of the MAP kinase (MAPK) family, and is activated by MAP/ERK kinase 1 (MEK1) and MEK2. ERK1 and ERK2, are emerging as important regulators of neuronal responses to both functional and pathologic stimuli.
A number of recent studies in models of cerebral ischemia, brain trauma and neurodegenerative diseases implicate a detrimental role for ERK1/2 signalling during oxidative neuronal injury.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/4000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1062 detects a band of approximately 45kDa in human brain, HEK293 and A431lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to ERK 1 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
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