AHP1060 detects an epitope within the C-terminal (CT) region of cyclin-dependent kinase inhibitor 2A (CDKN2A). CDKN2A is a 16kD protein which belongs to the CDKN2 cyclin dependent kinase inhibitor family. It acts as a negative regulator of cell proliferation by interacting strongly with CDK4 and CDK6, thus inhibiting their ability to interact with cyclin D and to phosphorylate the retinoblastoma protein. CDKN2A is an important tumour suppressor and defects in its production results in tumour formation in a wide range of tissues, including cutaneous malignant melanoma and Li-Fraumeni syndrome.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32,000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1060 detects a band of approximately 16-18kDa in A431 and Hela cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to CDKN2A (isoform 1) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
京公网安备11010802025653