GOAT ANTI HUMAN CD154

产品编号：AHP1029 品牌：AbD Serotec 原厂货号：AHP1029
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

0.1 mg

运保温度:

+4oC or -20oC

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描述：

Specificity

AHP1029 is specific for the soluble form of human CD154, also designated CD40L (sCD40 Ligand), a member of the TNF receptor superfamily, comprising the full-length of the TNF homologous region of CD154.

CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells.

Species Cross-Reactivity

Target Species	Cross Reactivity
Yeast	Yes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
ELISA				0.5 - 2.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin⁽¹⁾				2.5 - 5.0ug/ml
Immunoprecipitation
Western Blotting				0.1 - 0.2ug/ml

(1)

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Storage

Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of reconstitution.

Antiserum Preparation

Antisera to human CD154 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.