AHP1023 recognises human TOLLIP or Toll interacting protein. TOLLIP is a 30kDa adaptor molecule for Toll-like receptors, transducing signals and activating kinases and transcription factors in response to an infection. It is thought to inhibit IRAK-1 activity. This facilitates the termination of the IL-1RI-, TLR2- and TLR4-triggered, NF-kB and JNK signalling pathways during inflammation. Furthermore, TOLLIP is also thought to form a complex with Tom1, which regulates endosomal trafficking of ubiquitinated proteins. Unlike most other inflammation suppressors, TOLLIP is constitutively expressed.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1023 detects a band of approximately 30kDa in rat brain cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to TOLLIP were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
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