AHP1022 recognises human CD289, otherwise known as toll receptor 9, a member of the evolutionarily conserved toll-like receptor family (TLRs), which play an important role in the recognition of invading microbial pathogens during innate immunity.
CD289 is expressed preferentially by the spleen, lymph node, bone marrow and peripheral blood leucocytes and recognises viral DNA and unmethylated CpG sequences in bacterial DNA. Unlike other TLRs, TLR9 is dependant upon the adaptor molecule MyD88 for signaling activation.
AHP1022 has been shown as suitable for use in IHC on human Jurkat cell cytospins @ 2ug/ml.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1022 detects a band of approximately 95kDa in human Jurkat cell lysates . (Predicted Mwt. 115-120kDa).
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human CD289 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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