AHP1005 recognises an epitope within the C-terminal region (CT) of the 244 amino acid protein Adiponectin (also known as Acrp30), a major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism.
Unlike most other adipokines, Adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The serum level of Adiponectin is inversely correlated with BMI (body mass index) of an individual, and has an anti-inflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Yes
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
10ug/ml
Immunoprecipitation
Western Blotting
1 - 2ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1005 detects a band of approximately 26.4kDa in human HL60 cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Human Adiponectin (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
京公网安备11010802025653